Figure 7. CAV1 silencing precludes the increase in cell migration induced by Methotrexate and Etoposide in colon cancer cell lines.
Parental, sh-Scramble and sh-CAV1 (#5) (A) HT29(US) or (B) DLD-1 cells (6 x 105) were seeded in 6 cm plates 24 h before treatment with 100 nM Methotrexate or 10 μM Etoposide for 48 h. Cells (2 x 105) were then seeded in Boyden chambers coated with fibronectin (2 μg/ml) on the lower side and allowed to migrate for 7 h (HT29(US) cells) or 5 h (DLD1 cells). The cells that migrated through the pores were stained and counted. Values obtained were normalized to those obtained for parental cells without treatment. The graphs show the averages of values from 3 independent experiments (mean ± SEM). Significant differences are indicated **p ≤ 0.01, *p ≤ 0.05.