Table 5. Known variants in paediatric cancer cell lines were compared against capture sequencing from the Cancer Cell Line Encyclopaedia and other published data.

Cell line ID	Gene	Alteration	Detected	Allele frequency expected	Allele frequency observed
Be(2)C	TP53	p.C135F	YES	no data available	100%
Be(2)C	MYCN	AMPLIFICATION	YES	not applicable	not applicable
CCA	KRAS	p.Q61L	YES	no data available	29%
IMR32	ATM	p.V2716A	YES	59%	59%
IMR32	MYCN	AMPLIFICATION	YES	not applicable	not applicable
KELLY	ALK	p.F1174L	YES	39%	32%
KELLY	MAP2K1	p.A390T	YES	48%	47%
KELLY	TP53	p.P177T	YES	93%	99%
KELLY	MYCN	AMPLIFICATION	YES	not applicable	not applicable
LAN1	ALK	p.F1174L	YES	no data available	47%
LAN1	TP53	p.C182*	YES	no data available	99%
LAN1	MYCN	AMPLIFICATION	YES	not applicable	not applicable
LAN5	ALK	p.R1275Q	YES	no data available	50%
LAN5	MYCN	AMPLIFICATION	YES	not applicable	not applicable
NBLS	NF1	splice_acceptor_variant c.6705-1G>T	YES	no data available	42%
RD	ATM†	p.D273N	YES	17%	2%
RD	NF1	p.E977*	YES	56%	59%
RD	NRAS	p.Q61H	YES	68%	61%
RD	TP53	p.R248W	YES	100%	100%
RH30	CDK4	AMPLIFICATION	YES	not applicable	not applicable
RH41	APC	p.M526L	YES	60%	59%
RH41	TP53	p.P152fs	YES	100%	100%
RMS559	FGFR4	p.V582L	YES	no data available	76%
SKNAS	NRAS	p.Q61L	YES	45%	46%
SKNAS	RB1	p.L477P	YES	47%	31%
SKNAS	TP53	DEL exons 10,11	YES	not applicable	not applicable
SKNSH	NRAS	p.Q61L	YES	15%	23%
SKNSH	SMARCA4	p.R973T	YES	32%	45%
SKNSH	CHEK2	p.T410fs	YES	59%	44%
SKNSH	ALK	p.F1174L	YES	no data available	36%

^†ATM mutation in this cell line is subclonal and variation in AF is expected with on-going passages.