Skip to main content
. Author manuscript; available in PMC: 2019 Jan 9.
Published in final edited form as: Cell Metab. 2017 Nov 2;27(1):167–179.e7. doi: 10.1016/j.cmet.2017.10.003

Figure 1. 2 week-OVX decreases mitochondrial function and induces an oxidative shift in SM cellular redox environment.

Figure 1

(A) Citrate synthase activity in RG. (B) JO2 measured in PmFbs from RG. (C) Fatty acid-supported JO2. Abbreviations: glutamate/malate (G/M), succinate (Succ), rotenone (Rot), antimycin A (AmA), ascorbate (Asc), N,N,N′,N′-Tetramethyl-p-phenylenediamine dihydrochloride (TMPD), palmitoyl-carnitine (PC), almitoyl-CoA (P-CoA), L-carnitine (L-Carn). (D–F) Mitochondrial respiratory kinetics. Pyruvate titrations in the presence of malate and ADP (D), and ADP titrations in the presence of G/M (E) or pyruvate/malate (F). Kinetic parameters KM and Vmax were determined from fitting to Michaelis Menten functions. Changes in Vmax were significant (*p<0.05). (G) Total GSH and GSSG concentration and resulting GSH/GSSG ratios (H) in RG. (I) JH2O2 measured in PmFbs pre-incubated with or without 1-chloro-2,4-dinitrobenzene (CDNB) for GSH depletion, then added pyruvate. Values are means ± SEM; * p<0.05; ** p<0.005; *** p<0.0005, N = 8-15 mice/group.