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. Author manuscript; available in PMC: 2019 Feb 16.
Published in final edited form as: J Mol Biol. 2017 Jul 10;430(4):537–553. doi: 10.1016/j.jmb.2017.06.023

Figure 3.

Figure 3

DDX21 helicase activity is stimulated by ATP. a) Schematic diagram of RNA molecules used in helicase assay. A fluorescently labelled RNA 10mer is annealed to a fluorescent quencher 26mer. Once the strands separate, the 10mer reanneals to the excess unlabeled 14mer, resulting in a fluorescent signal. b) Helicase assay results showing a graph of fluorescent intensity over time for RNA dimer alone (open boxes), DDX21 with RNA dimer (closed boxes) and DDX21 with ATP and RNA dimer (closed circles).