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. Author manuscript; available in PMC: 2019 Feb 16.
Published in final edited form as: J Mol Biol. 2017 Jul 10;430(4):537–553. doi: 10.1016/j.jmb.2017.06.023

Figure 7.

Figure 7

DDX21 and Rev bind independently to the RRE. a) Electrophoretic gel mobility shift titration of DDX21 into radiolabeled RRE RNA (top panel), and quantified fraction bound graph (on bottom). See materials and methods for line fit of bottom panel. Kd is noted on bottom. b) EMSA titration of Rev (top panel), DDX21(83–620) (middle panel), and DX21(83–620) in the presence of 25 nM Rev (bottom panel) into RRE RNA; RRE band imaged via ethidium bromide staining and U.V. fluorescence. Rev-dependent species are marked by a solid line, and DDX21(83–620)-dependent species by dotted lines.