Figure 3.

Hpl proteins localize to macronuclear chromatin, new developing macronuclei, and pycnotic nuclei. YFP fusion proteins were expressed by induction with CdCl₂ and visualized by epifluorescence microscopy during regular growth, 5 h into conjugation, and 9 h into conjugation. Schematic at the top shows arrangements of various nuclei. DIC images on the left of each image pair show positions of nuclei. NS = not shown; NM = new macronucleus; mic = micronucleus; Mac = macronucleus; om = old macronucleus. Scale bars = 10 μm apply to all panels in the set.