Fig. 1. Effects of β-catenin on the growth and maintenance of squamous cell carcinoma (SCC) cells. (A) SCC13 cells were transduced with 10 multiplicity of infections of adenovirus expressing β-catenin for overnight, then fresh growth medium was added. Cells were further incubated for 2 days, then cellular extracts were prepared and analyzed by Western blot. Adenovirus (Ad) expressing LacZ was used as a negative control. (B) SCC13 cells were transduced with a recombinant adenovirus for overnight. After replenish with fresh medium, cells were further incubated in the presence of [³H]thymidine for 2 days. Radioactivity was measured using liquid scintillation counter. The data are represented as percent control. The mean values±standard deviation are averages of triplicate measurements. ^*p<0.01. (C) SCC13 cells were transduced with a recombinant adenovirus for overnight. Cells were then detached from culture dish and about 2,000 cells were seeded on 100-mm dish and cultured for 2 weeks.