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. 2016 Jan 25;6(3):137–149. doi: 10.1016/j.jpha.2016.01.003

Table 5.

Performance characteristics of TLC and HPTLC methods.

S.No. Stationary phase; Mobile phase Detection (nm) Linear range (µg/spot) LOD (µg/spot) LOQ (µg/spot) Application Reference

1 Spectrodensitometry 283 5–25 Pharmaceutical preparations containing TFH and triamcinolone [16]
2 Silica gel 60F254; 25% aqueous ammonia–chloroform–methanol (0.1:12:0.1, v/v/v) 284 Bulk drug, degradation products [35]
3 Silica gel 60F254; Formic acid-toluene–ethylacetate (0.1:4.5:5.5, v/v/v) 284 0.035 Pharmaceuticals, accelerated stability testing [39]
4 Silica gel 60F254; n-hexane: acetone: glacial acetic acid (8:2:0.1, v/v/v) 223 0.2–1 0.0012 0.0036 Bulk drug and tablets; SIAM [40]
5 Silica gel 60F254; acetonitrile: 1,4-dioxane: hexane: acetic acid (1:1:8:0.1, v/v/v/v) 282 0.5–5 0.30 0.39 Tablets [41]

SIAM: Stability-indicating assay method.