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. 2017 Aug 3;8(68):112390–112400. doi: 10.18632/oncotarget.19837

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Copyright: © 2017 Kim et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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(A, B) Immunocytochemistry staining for phosphorylated H2AX, a marker of DNA damage response, in Huh7 and HepG2 cells exposed to γ-rays (5 Gy) and neutron radiation (5 GyE) in the absence or presence of gold nanoparticles, assessed 30 min, 6 h and 24 h after irradiation. (C) Immunoblotting of cell lysates with indicated antibodies. The absorbed doses were 5 Gy for γ-rays and 5 GyE for neutrons. Band intensities for target proteins were normalized to that for β-actin. Values represent the means of 3 experiments ± SD.

(A, B) Immunocytochemistry staining for phosphorylated H2AX, a marker of DNA damage response, in Huh7 and HepG2 cells exposed to γ-rays (5 Gy) and neutron radiation (5 GyE) in the absence or presence of gold nanoparticles, assessed 30 min, 6 h and 24 h after irradiation. (C) Immunoblotting of cell lysates with indicated antibodies. The absorbed doses were 5 Gy for γ-rays and 5 GyE for neutrons. Band intensities for target proteins were normalized to that for β-actin. Values represent the means of 3 experiments ± SD.