Figure 1. Actein suppresses cell proliferation in human bladder carcinoma cell lines.

(A) Human bladder cancer cell lines of BIU-87, T24, T739 and 5637 were treated with different concentrations (0, 2.5, 5, 10, 20 and 40 uM) of ACT for 24 h or 48 h, followed by MTT analysis to calculate the cell viability. (B) Human normal bladder cell line of SV-HUC-1 and human normal liver cell line of L-02 were cultured with ACT at the indicated doses for 24, 48 or 72 h, and then the cell viability was measured using MTT analysis. (C) Human bladder cancer lines of BIU-87 and T24 were treated with different doses of ACT for 24 h, followed by clonogenic assays. Data are represented as mean ± S.E.M. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001 versus the untreated group.