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. 2017 Nov 8;8(68):112584–112597. doi: 10.18632/oncotarget.22576

Table 2. Real-time qPCR Primer.

Primer Sequence (5′ to 3′) Primer (bp) Amplicon (bp) Annealing temperature (°C)
PD-L1_2* s AGACCACCACCACCAATTCC 20 173 60
PD-L1_2* /as TGGAGGATGTGCCAGAGGTA 20
PD-L1_4** /s AGCTATGGTGGTGCCGACTA 20 152 60
PD-L1_4** /as CAGATGACTTCGGCCTTGGG 20
GAPDH in /s GACCCCTTCATTGACCTCAACTA 23 102 60
GAPDH in /as GAATTTGCCATGGGTGGAAT 20

*amplification of transcript variant 1 and 2 simultaneously; amplicon named PD-L1_2

**amplification of transcript variant 1 and 4 simultaneously; amplicon named PD-L1_4

The selected primers for RT-qPCR mRNA expression analyses of PD-L1_4 (including PD-L1 splicing variants 1 and 4) and PD-L1_2 (including PD-L1 splicing variants 1 and 2).