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. 2017 Nov 8;8(68):112584–112597. doi: 10.18632/oncotarget.22576

Table 3. PD-L1 expression in tissue and peripheral blood of healthy controls and OSCC patients.

n mean ΔCT SD p-value AUC FC COP No. of cases + - % pos. cases p-value χ2 test
PD-L1_4 tissue 78 < 0.001 0.83 3.19 7.99 78 41 37 < 0.001
controls 35 8.82 1.11 35 6 29 17.1%
patients 43 7.15 1.59 43 35 8 81.4%
PD-L1_4 blood 75 0.165 nd nd nd nd nd nd nd nd
controls 31 7.17 1.40
patients 44 6.90 1.24
PD-L1_2 tissue 74 < 0.001 0.76 3.18 10.37 74 40 34 < 0.001
controls 31 10.92 1.45 31 8 23 25.8%
patients 43 9.25 1.94 43 32 11 74.4%
PD-L1_2 blood 75 0.287 nd nd nd nd nd nd nd nd
controls 31 7.10 1.33
patients 44 6.92 1.21

Comparison of PD-L1 mRNA expression between OSCC patients (group patients) and healthy volunteers (group controls). Expression of PD-L1 splicing variants 1 and 4 (PD-L1_4) and splicing variants 1 and 2 (PD-L1_2) in tissue (OSCC tumor tissue vs. healthy oral mucosa of volunteers) and peripheral blood was analyzed. The mean ΔCT value (mean), standard deviation (SD) and the p-value provided by the Mann-Whitney U test are shown. Higher ΔCT values indicate lower PD-L1 mRNA expression. Regarding PD-L1 expression in tissue, area under the curve (AUC), fold-change FC and cut-off point (COP) values are given. Based on their ΔCT value related to the COP, the cases were determined as positive (malignant) and negative (healthy). The percentage of positive tested cases (% pos. cases) in the controls and patients group is presented. A Statistical analysis was carried out by the Chi-square test (χ2 test). Fold-change (FC) of PD-L1 mRNA expression was determined by the ΔΔCT method comparing the average ΔCT values of the two groups. nd: not determined.