Figure 4. NEIL3 is associated at replication fork during replication stress and its loss impaired the recruitment of replication associated homologous repair proteins.

(A) The recruitment of NEIL3, Rad51, RPA, PCNA, and CHK1 after HU treatment at new synthesized DNA fragments; (B) Schematic representation of replication fork recovery and the recruitment of homologous recombination proteins at the newly synthesized DNA after replication stress (2mM HU); (C) Protein bound to immunoprecipitation (ChIP) with IdU antibody after replication stress; (D) Chromatin associated fractions; (E) Nuclear fraction; (F) Base excision repair proteins recruited at newly synthesized DNA after replication stress in NEIL3 proficient and deficient cells; (G) Replication licensing proteins recruitment in NEIL3 proficient and deficient cells. No statistically significant difference was observed between NEIL3 proficient and deficient cells in data F and G.