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. 2017 Dec 4;8(68):112942–112958. doi: 10.18632/oncotarget.22896

Figure 6. Loss of NEIL3 confers sensitivity to ATR inhibition and synergizes with PARP1 inhibitor.

Figure 6

Estimated percent of clonogenic survival of NEIL3 proficient versus NEIL3 deficient cells with different concentration of Olaparib, ATR inhibitor and in combination (ATR inhibitor plus Olaparib (10nM)). (A-C) LN428 treated with Olaparib only (B) ATR inhibitor only (C) combination of ATR inhibitor and olaparib; (D) Western blot for knockdown of NEIL3 in LN428 cells; (E-G) LN18 NEIL3 proficient versus deficient cells treated with Olaparib; (H) Western blot for NEIL3 knockdown in LN18 cells (I); Western blot for Chk1 phosphorylation (Ser317) and Chk1 after ATR inhibition. Note that the ratio of the Chk1 (Ser317) versus total Chk1 signals for each lane are provided as an estimate of the relative stoichiometry of Chk1 Ser317 phosphorylation in each fraction; (J) Replication speed before and after ATR inhibitor in LN428 cells.