Skip to main content
. 2017 Dec 6;8(68):112980–112991. doi: 10.18632/oncotarget.22945

Figure 2. ATM is a miR-203a target gene.

Figure 2

(A) Protein lysates were prepared from MT330 GBM cells that were transduced with miR-203a-encoding or empty-vector (EV) lentivirus, and immunoblotted as indicated. (B) Sequence alignment of the miR-203a binding sequence with the 3'UTR of the wild-type (WT) and mutant (mut) ATM constructs used in the following reporter assays. 293T (C), SJG2 (D) and MT330 (E) cells were transiently cotransfected with miR-203a plasmid, pSV40-Renilla, and with plasmid empty-vector (pcDNA3.1-Luc), WT (pcDNA3.1-Luc-wtUTR) or mutant (pcDNA3.1-Luc-muUTR) ATM reporter plasmids. The ratio of luciferase and Renilla activities was determined at 24 hr post-transfection.