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. 2016 May 24;6(4):207–213. doi: 10.1016/j.jpha.2016.05.006

Table 1.

Different bioassay designs for agar diffusion assay.

Assay design Dose ratio Pharmacopoeia Informative statistical parameters References
2×2 2:1, 4:1 Indian Pharmacopoeia, British Pharmacopoeia, Brazilian Pharmacopoeia Regression significance, Parallelism [21]
3×3 2:1 Indian Pharmacopoeia, British Pharmacopoeia, Brazilian Pharmacopoeia Regression significance, Parallelism [28]
3×1 2:1 European Pharmacopoeia Regression significance, Linearity [21]
5×1 4:5 Indian Pharmacopoeia, United States Pharmacopoeia Regression significance, Linearity [21], [27]