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. 2018 Jan 10;8:304. doi: 10.1038/s41598-017-18307-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Augmentation of contractions in rat urinary bladder strips by pyridostigmine. (A) Typical contractions of rat urinary bladder strip induced by acetylcholine (100 μM) in control (left) and after inhibition of cholinesterases by 1 μM of pyridostigmine (right). (B) Dose-dependent effect of different pyridostigmine concentrations (1–1000 nM) on amplitude of ACh-induced contractions. Data are presented as mean ± SEM pooled from ten urinary bladder strips in each group. Asterisk (*) indicates statistically significant difference (p < 0.05).