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. 2018 Jan 10;8:304. doi: 10.1038/s41598-017-18307-9

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of inhibitors of cholinesterase activity on contractility of human urinary bladder strips. The bar graph represents the effects of iso-OMPA (50 μM), C547 (1 and 5 nM) and pyridostigmine (1 μM) on contractions produced by acetylcholine (100 μM) in human urinary bladder strips. Data are presented as mean ± SEM pooled from four bladder strips in each group, obtained from ten patients. Asterisk (*) indicates a statistically significant difference (p < 0.05) from control. Cross sign (+) indicates statistically significant difference (p < 0.05) in effects of combined application of iso-OMPA (50 μM) and C547 (1 and 5 nM) and application of any given single C547 concentration of C547. Hash/pound sign (#) indicates statistically significant difference (p < 0.05) in effects of C547 (5 nM) and pyridostigmine (1 μM).