Figure 4.

Quantitative analysis of EPS production in D. zeae wild-type strain EC1 and derivatives. Ten mililiters of overnight bacterial cultures in LB medium at a density of OD₆₀₀ = 1.8 were centrifuged at 12,000 rpm for 15 min. Two volumes (20 ml) of absolute ethanol were added into the collected supernatants and thoroughly mixed before incubation for 1 h at 4 °C. EPS was isolated by centrifuging at 12,000 rpm for 30 min at 4 °C and dried overnight at 55 °C before determination of the dry weights. Final results of fis mutant were normalized to that of the wild-type EC1, which was set to a value of 100%, for easy comparison. Experiments were repeated at least three times in triplicates. *P < 0.05 (Student’s t-test).