Table 2.
Strains and plasmids used in this study.
| Strains or plasmids | Relevant phenotypes and characteristicsa | Source or reference |
|---|---|---|
| Strains | ||
| EC1 | Wild type of Dickeya zeae, PBr | Lab collection |
| Δfis | A deletion mutant derived from EC1 | This research |
| Δfis(fis) | Δfis containing fis coding region at the downstream of lacZ promoter, Ampr, PBr | This research |
| CC118λ | Escherichia coli strain as host for plasmid constructs derived from pKNG101 | Lab collection |
| DH5α | E. coli strain as host for plasmid constructs derived from pBBR1-MCS4 | Lab collection |
| HB101(pRK2013) | Thr leu thi recA hsdR hsdM pro, Kanr | Lab collection |
| Plasmids | ||
| pKNG101 | Knockout vector, Strr | Lab collection |
| pKNG101-fis | pKNG101 carries the in-frame deleted fragment of fis, Strr | This research |
| pGEX-6p-fis | pGEX-6p-1 carries the fis coding region, Ampr | This research |
| pBBR1-MCS4 | Expression vector contains a lacZ promoter, Ampr | This research |
| pBBR1-fis | pBBR1-MCS4 carries the coding region of fis at down-stream of lacZ promoter, Ampr | This research |
aPBr, Ampr, Kanr, Strr = resistance to Polymyxin B Sulfate, Ampicillin, Kanamycin, or Streptomycin, respectively.