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. 2018 Jan 10;8:327. doi: 10.1038/s41598-017-18612-3

Figure 2.

Figure 2

MiR655 over-expression promotes EMT in MCF7 cells and miR655 knock-down in MCF7-COX2 cells reverts to MET. Fold change in E-Cadherin, N-Cadherin (CDH2), ZEB1, Vimentin and SNAIL mRNA (A) and protein expression (except ZEB1 and SNAIL) (B) in MCF7-miR655 cells indicating EMT. Immunofluorescence assay showing down regulation of E-Cadherin (C) and upregulation of TWIST1 (D) in MCF7-miR655 cells. Knock down of miR655 in MCF7-COX2 cells up regulates E-Cadherin and down regulates Vimentin mRNA (E) and protein, measured with Western Blots (F), quantification presented in (G). For quantification, all comparisons were done relative to respective Mock cell lines. In figures (B) and (F), each marker was run on separate western blot and full western blots for Vimentin and E-Cadherin are shown in Supplementary Figures 3 & 4 respectively. Quantitative data are presented as a mean of triplicate independent experiments ± SEM. *Indicates p < 0.05, **p < 0.01 compared to parental and mock cell lines. Scale bar is 100 µm.