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. 2018 Jan 10;8:327. doi: 10.1038/s41598-017-18612-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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MiR655 over-expression induces stem-like cell phenotype in MCF7 and SKBR3 cells. (A) MiR655 expression tested in spheroids relative to monolayer culture conditions in breast cancer cell lines. (B) Comparison of spheroid formation by miR655 overexpressing cells and respective control cells presented as spheroid numbers (C) and sizes (perimeter). (C) Expression of NOTCH pathway genes (NOTCH1, 2, 3) and (D) WNT genes (AXIN1, AXIN2, CCDN1 and c-MYC) in MCF7-miR655 cells. We identified SLC marker positive cells ALDH (F) and OCT3/4 (G) in MCF7 and MCF7-miR655 cells with immunofluorescence assay. Incidence of marker positive cells is presented in (H). Quantitative data are presented in as a mean of triplicate independent experiments ± SEM. *Indicates p < 0.05, **p < 0.005 and ***p < 0.001 compared to parental or mock or monolayer cell lines. Scale bar is 60 µm.