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. 2018 Jan 10;8:309. doi: 10.1038/s41598-017-18701-3

Figure 7.

Figure 7

8u could inhibit the expression of FASN and inactivation of the PI3K/Akt pathway. (A) Integrated pathway analysis using Metaboanalyst. 8u mainly affected fatty acid biosynthesis. (B) Western blotting analysis of FASN protein expression after cell exposure (or not) to the shown concentrations of 8u for 48 h. (C) The densitometry of FASN protein performed on the western blotting of B. (D) Cells were treated with 8u at indicated concentrations for 24 h, and the expression of p-Akt and Akt proteins were determined by western blotting. (E) Quantification of p-Akt/Akt ratio were performance according to the western blotting results. (F) HepG2 Cells were pretreated for 2 h with or without 20 μM LY294002 and then with 8u (6 μM) for an additional 24 h. The phosphorylation of AKT was measured by western blotting. (G) The densitometry of p-Akt protein performed on the western blotting of F. (H) HepG2 cells were pretreated for 2 h with or without 20 μM LY294002 and then with DMSO for an additional 24 h. The FASN protein was measured by western blotting. (I) Densitometry of FASN protein performed on the western blotting of H. All the western blotting data presented were means ± SD of three independent experiments, and the significant difference was set at p < 0.05. *p < 0.05, **p < 0.01 compared with the control group.