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. 2018 Jan 10;8:233. doi: 10.1038/s41598-017-18665-4

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Scheme of synthesis for the hemostatic THR@ferria colloid. After mixing the Fe² ⁺/Fe³ ⁺ salts, an ammonia solution is added (a) followed by washing the precipitate of the formed magnetite (b) and treating it ultrasonically to yield a stable hydrosol (c). Then, the solution of thrombin (d) is added to the sol and the system is condensed by removal of the solvent and physical gelation. The resulting composite with entrapped thrombin molecules is ground (e) and a hemostatic colloid is prepared by filtering the ground nanoparticles through a filter with a nozzle of 200 nm.