Figure 2.

Microwell platform manufacture and establishment of 3D micro-spheroid culture. (a) The traditional open-top microwell platform results in dislodged spheroids following culture media exchange (scale bar = 500 µm). (b) Schematic illustration and bright field images show PDMS discs with and without the mesh which can be inserted in 48-well tissue culture plates and cells aggregated after 24 hours of seeding cell suspension (scale bar = 200 µm). (c) Schematic illustration of cell seeding using traditional open-top microwells (top) and Microwell-mesh being microwells modified with a 36 µm mesh-top (bottom) large enough to allow single cells to pass through, but small enough to retain spheroids within discrete microwells.