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. 2018 Jan 10;8:284. doi: 10.1038/s41598-017-18849-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Confocal laser scanning microscope (CLSM) images of Daphnia magna that were not exposed to nano-sized polystyrene (nPS; green emissions) (a) and that were exposed through the diet (b,c). Z-stack image of individual exposed through the diet (b), observed using CLSM (d). Z-stack image (d) provides evidence of nPS uptake through dietary exposure of D. magna. Green fluorescent nPS was observed in the gut of exposed D. magna. Scale bar = 200 μm.