Table 1.
Calculated and observed [M + H+] ions of synthetic analogs of Aβ and ratAβ metal-binding domains and their cleavage products generated by the action of N-ACE and C-ACE.
| Amyloid peptide | Peptide sequence | Calculated m/z | Mean Observed m/z |
|---|---|---|---|
| Substrates | |||
| Aβ(1-16) | DAEFRHDSGYEVHHQK | 1954.87906 | 1954.8 |
| Aβ(1-16)-[Amide] | DAEFRHDSGYEVHHQK-[Amide] | 1953.89505 | 1953.9 |
| [Acetyl]-Aβ(1-16)-[Amide] | [Acetyl]- DAEFRHDSGYEVHHQK Amide] | 1995.90561 | 1995.8 |
| [Acetyl]-ratAβ(1-16)-[Amide] | [Acetyl]-DAEFGHDSGFEVRHQK-[Amide] | 1899.87325 | 1899.9 |
| Products | |||
| Aβ(1-14) | DAEFRHDSGYEVHH | 1698.72552 | 1698.6 |
| Aβ(1-13) | DAEFRHDSGYEVH | 1561.66661 | 1561.5 |
| Aβ(6-16)-[Amide] | HDSGYEVHHQK-[Amide] | 1335.61887 | 1335.6 |
| [Acetyl]-Aβ(1-5) | [Acetyl]- DAEFR | 679.30458 | 679.3 |
| [Acetyl]-ratAβ(1-15) | [Acetyl]-DAEFGHDSGFEVRHQ | 1772.76230 | 1772.9 |
| [Acetyl]-ratAβ(1-14) | [Acetyl]-DAEFGHDSGFEVRH | 1644.70372 | 1644.9 |
| [Acetyl]-ratAβ(1-13) | [Acetyl]-DAEFGHDSGFEVR | 1507.64481 | 1507.5 |