Fig. 1.

Pex15 inhibits the ATPase activity of Pex1/Pex6 in a pore-loop-dependent manner. a ATPase activity of wild-type (WT) and D2 pore-loop (PL2) mutant Pex1/Pex6 alone and in the presence of Pex15, Ubp15, Pex5, or a linear ubiquitin-Pex5 fusion (mean and s.d. for n = 3 technical replicates). The D2 pore loop mutations are Pex1-F771A and Pex6-Y805A. b Pex15’s cytosolic domain binds both wild-type Pex1/Pex6 and the Pex1/Pex6 D2 pore-loop mutant. c Pore-loop mutations in either Pex1 or Pex6 reduce the inhibitory effect of Pex15. ATPase data shown are the mean±s.d. for n = 3 technical replicates