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. 2018 Jan 10;9:135. doi: 10.1038/s41467-017-02474-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — Pex15 bridges Pex5/Pex14 with Pex1/Pex6. a A pull-down binding assay of GST-Pex14 with Pex5, Pex15 1-327-FLAG, and Pex1/Pex6 in the presence of ATP shows that Pex1/Pex6 associates indirectly with Pex14 through an interaction requiring Pex5 and Pex15. Pex15-FLAG was detected by an anti-FLAG western blot on the input and elution. b A pull-down binding assay of MBP-Pex15 1-327 with Pex1/Pex6-WB2 and fluorescein labeled Pex5 with and without a PTS1 peptide. MBP-Pex15 and Pex5 migrate similarly, so the presence of Pex5 was determined by imaging its fluorescein label. MBP alone does not bind Pex5 (Supplementary Figure 9)