Table 1.
Glycerol fermentation in single- and co-culture of Escherichia coli with Methanobacterium formicicum
Consumed (mM) | Produced (mM) | Cell number (mL−1) | |||||
---|---|---|---|---|---|---|---|
Glycerol | Succinate | Formate | Acetate | Ethanol | E. coli | M. formicicum | |
Single culture | |||||||
E. coli | 4.6 ± 1.6 | 1.0 ± 0.1 | 10.7 ± 3.5 | 1.7 ± 0.9 | 9.6 ± 2.0 | 3.4 × 108 ± 0.4 × 108 | ND |
Co-culture | |||||||
E. coli
M. formicicum |
53 ± 12.8a | 8.0 ± 0.7a | 0 | 7.3 ± 1.0 | 47.2 ± 16.1 | 1.1 × 109 ± 0.2 × 109 | 2.1 × 108 ± 1.2 × 108 |
Product analysis and cell growth were determined after 7 days of fermentation. Values report means ± standard deviations for three replicates
aValue means a significant difference between single- and co-culture (unpaired samples t test, P < 0.05). ND, not determined