Fig. 1.

Somatic hotspot mutations in FGFR1. a Overview of next-generation sequencing reads from the mutated sites in Integrative Genomics Viewer. Read bases that match the hg19 reference are displayed in gray, and mismatches are indicated with color coded alternate alleles (FGFR1 is oriented on the reverse strand; hence, the sequence is here the reverse complement to the transcribed sequence). b Validation of the mutations in the two tumor samples (64T and 40T) with Sanger sequencing (in the direction of transcription) and the corresponding sequences from blood samples