Table 1.
Compounds identified in the extracts of females and males of Leptopilina clavipes. KRI = Kovats retention index on a non-polar (DPX-5) GC column. Diagn. Ion = diagnostic ions used in the identification of the compound. Diag. Ion DMDS = diagnostic ions of unsaturated compounds after derivatisation with DMDS. The percentage of compounds is based on the total peak area of all identified peaks. tr = trace amounts. Numbers of compounds correspond to Fig. 1.
| No. | Compound | KRI | Diagn. Ions | Diagn. Ions DMDS | Females Mean % | Females SD % | Males Mean % | Males SD % |
|---|---|---|---|---|---|---|---|---|
| 1 | Iridodial 1 | 1313 | 168 (M+), 111, 135 | 1.07 | 0.52 | 0.13 | 0.13 | |
| 2 | Iridodial 2 | 1317 | 168 (M+), 109, 135 | 0.75 | 0.37 | 0.12 | 0.11 | |
| 3 | unknown, identical to P3 in13 | 1322 | 67, 81, 109, 152 | 0.51 | 0.23 | 0.24 | 0.14 | |
| 4 | unknown, identical to P4 in13 | 1365 | 67, 81, 109 | 0.38 | 0.28 | 0.22 | 0.28 | |
| 5 | (−)-iridomyrmecin | 1467 | 168 (M+), 95, 109 | 14.81 | 5.65 | 2.89 | 1.31 | |
| 6 | (+)-isoiridomyrmecin | 1479 | 168 (M+), 95, 109 | 0.23 | 0.09 | tr | ||
| 7 | Heptadecane | 1700 | 240 (M+) | 0.19 | 0.07 | 0.04 | 0.02 | |
| 8 | Octadecane | 1800 | 254 (M+) | 0.06 | 0.03 | 0.04 | 0.02 | |
| 9 | Nonadecane | 1900 | 268 (M+) | 0.10 | 0.04 | 0.05 | 0.03 | |
| 10 | Heneicosane | 2000 | 282 (M+) | 0.10 | 0.03 | 0.07 | 0.03 | |
| 11 | Eicosane | 2100 | 296 (M+) | 0.08 | 0.02 | 0.06 | 0.05 | |
| 12 | Docosane | 2200 | 310 (M+) | 0.03 | 0.01 | 0.03 | 0.04 | |
| 13 | 4-methyl docosane | 2262 | 324 (M+), 309 (M-15), 281 | 0.36 | 0.32 | 0.06 | 0.06 | |
| 14 | 9-tricosene | 2275 | 322 (M+), 97 | 416 (M+), 173, 243 | 0.30 | 0.23 | 0.04 | 0.03 |
| 15 | 7-tricosene | 2282 | 322 (M+), 97 | 416 (M+), 145, 271 | 0.09 | 0.07 | ||
| 16 | Tricosane | 2300 | 324 (M+) | 0.10 | 0.06 | 0.05 | 0.05 | |
| 17 | Tetracosane | 2400 | 338 (M+) | 0.05 | 0.03 | |||
| 18 | 4-methyl tetracosane | 2463 | 352 (M+), 337 (M-15), 309 | 1.20 | 0.83 | 0.74 | 0.51 | |
| 19 | x,x-pentacosadiene1 | 2473 | 348 (M+), 96 | 0.23 | 0.16 | 0.12 | 0.05 | |
| 20 | 9-pentacosene | 2476 | 350 (M+), 97 | 444 (M+), 173, 271 | 1.13 | 0.67 | 1.86 | 0.64 |
| 21 | 7-pentacosene | 2483 | 350 (M+), 97 | 444 (M+), 145, 299 | 0.48 | 0.27 | ||
| 22 | Pentacosane | 2500 | 352 (M+) | 0.56 | 0.37 | 0.07 | 0.04 | |
| 23 | Hexacosane | 2600 | 366 (M+) | 0.19 | 0.14 | tr | ||
| 24 | 4-methyl hexacosane | 2663 | 380 (M+), 365(M-15), 337 | 2.26 | 1.31 | 0.64 | 0.33 | |
| 25 | 9-heptacosene | 2677 | 378 (M+), 97 | 472 (M+), 173, 299 | 0.99 | 0.63 | tr | |
| 26 | 7-heptacosene | 2685 | 378 (M+), 97 | 472 (M+), 145, 327 | 3.91 | 2.32 | 0.12 | 0.07 |
| 27 | Heptacosane | 2700 | 380 (M+) | 1.09 | 0.74 | tr | ||
| 28 | 13-methyl heptacosane and 11-methyl heptacosane | 2730 | 379 (M-15), 196/197, 224/225 and 379 (M-15), 168/169, 252/253 | 0.22 | 0.08 | 0.03 | 0.01 | |
| 29 | 5-methyl heptacosane | 2748 | 379 (M-15), 337, 85 | 0.03 | 0.02 | 0.09 | 0.02 | |
| 30 | 4-methyl heptacosane | 2763 | 394 (M+), 379 (M-15), 351 | 0.17 | 0.05 | 0.12 | 0.07 | |
| 31 | Octacosane | 2800 | 394 (M+) | 0.40 | 0.07 | 0.18 | 0.16 | |
| 32 | x-methyl x-octacosene1 | 2832 | 406 (M+), 97 | 0.28 | 0.11 | 0.12 | 0.07 | |
| 33 | x,x-nonacosadiene1 | 2854 | 404 (M+), 96 | 0.37 | 0.21 | |||
| 34 | 4-methyl octocosane | 2862 | 408 (M+), 393 (M-15), 365 | 11.67 | 1.54 | 7.55 | 1.15 | |
| 35 | 9-nonacosene | 2877 | 406 (M+), 97 | 500 (M+), 173, 327 | 1.00 | 0.50 | 0.58 | 0.43 |
| 36 | 7-nonacosene | 2886 | 406 (M+), 97 | 500 (M+), 145, 355 | 1.71 | 1.01 | tr | |
| 37 | Nonacosane | 2900 | 408 (M+) | 1.02 | 0.55 | 1.07 | 1.23 | |
| 38 | 15-methyl nonacosane and 13-methyl nonacosane | 2926 | 407 (M-15), 224/225 & 407 (M-15), 196/197, 252/253 | 0.76 | 0.16 | 0.10 | 0.04 | |
| 39 | 5-methyl nonacosane | 2946 | 422 (M+), 407 (M-15), 85, 365 | 0.19 | 0.04 | 0.06 | 0.02 | |
| 40 | 4-methyl nonacosane | 2960 | 422 (M+), 407 (M-15), 379 | 0.68 | 0.12 | 0.44 | 0.15 | |
| 41 | 3-methyl nonacosane and 5,x-dimethyl nonacosane1 | 2973 | 422 (M+), 407 (M-15), 393 & 421 (M-15), 379 | 0.91 | 0.28 | 0.28 | 0.14 | |
| 42 | Triacontane | 3000 | 423(M+) | 0.30 | 0.16 | 0.09 | 0.06 | |
| 43 | x-methyl x-triacontene1 | 3028 | 434 (M+) | 0.84 | 0.26 | 0.24 | 0.13 | |
| 44 | x,x-hentriacosadiene1 | 3045 | 432 (M+), 96 | 1.85 | 0.60 | 0.19 | 0.12 | |
| 45 | x,x-hentriacosadiene1 | 3053 | 432 (M+), 96 | 0.23 | 0.10 | tr | ||
| 46 | 4-methyl triacontane | 3060 | 436 (M+), 421 (M-15), 393 | 19.63 | 2.40 | 14.25 | 5.08 | |
| 47 | 15-hentriacontene & 14-hentriacontene | 3064 | 434 (M+), 97 | 528 (M+), 257, 271 & 528 (M+), 243, 285 | 3.09 | 1.92 | ||
| 48 | 9-hentriacontene | 3078 | 434 (M+), 97 | 528 (M+), 173, 355 | 1.87 | 0.86 | 0.69 | 0.37 |
| 49 | x-hentriacontene1 (probably 7-hentriacontene) | 3087 | 434 (M+), 97 | 0.23 | 0.13 | |||
| 50 | Hentriacontane | 3100 | 436 (M+) | 0.54 | 0.34 | 0.21 | 0.10 | |
| 51 | unknown | 3110 | 0.05 | 0.04 | ||||
| 52 | unknown | 3117 | 0.12 | 0.08 | ||||
| 53 | 15-methyl hentriacontane & 13-methyl hentriacontane | 3125 | 435 (M-15), 224/225, 252/253 & 435 (M-15) 196/197, 280/281 | 2.69 | 1.08 | 0.79 | 0.35 | |
| 54 | 5-methyl hentriacontane | 3145 | 435 (M-15), 85, 393 | 0.23 | 0.10 | 0.24 | 0.12 | |
| 55 | 7,15-dimethyl hentriacontane | 3161 | 449 (M-15), 435 (M-30), 112/113, 252/253, 239, 379 | 1.25 | 0.42 | 0.72 | 0.35 | |
| 56 | 5,17-dimethyl hentriacontane | 3172 | 449 (M-15), 435 (M-30), 84/85, 224/225, 267, 407 | 2.32 | 1.16 | 0.84 | 0.58 | |
| 57 | unknown | 3191 | 0.15 | 0.07 | ||||
| 58 | Dotriacontane | 3200 | 450 (M+) | 0.38 | 0.25 | 0.18 | 0.11 | |
| 59 | x-methyl x-dotriacontene1 | 3227 | 462 (M+) | 0.68 | 0.23 | 0.32 | 0.20 | |
| 60 | x,x-tritriacontadiene1 | 3245 | 460 (M+), 96 | 2.43 | 0.59 | 2.74 | 0.74 | |
| 61 | x,x-tritriacontadiene1 | 3253 | 460 (M+), 96 | 0.76 | 0.14 | 3.48 | 1.07 | |
| 62 | 4-methyl dotriacontane | 3259 | 464 (M+), 449 (M-15), 421 | 3.10 | 0.39 | 1.50 | 0.60 | |
| 63 | 16-tritriacontene | 462 (M+), 97 | 556 (M+), 271, 285 | 2.46 | 0.66 | |||
| 64 | x-tritriacontene1 (probably 9-tritriacontene) | 3279 | 462 (M+), 97 | 0.78 | 0.43 | 0.41 | 0.11 | |
| 65 | x-tritriacontene1 (probably 7-tritriacontene) | 3287 | 462 (M+), 97 | 0.19 | 0.10 | tr | ||
| 66 | Tritriacontane | 3300 | 464 (M+) | 0.12 | 0.15 | |||
| 67 | x-methyl x-tetratriacontene1 | 3315 | 476 (M+), 97 | 570 (M+), 131, 439 (prob. 6-en) | 0.70 | 0.36 | ||
| 68 | 17-methyl tritriacontane & 15-methyl tritriacontane & 13-methyl tritriacontane | 3324 | 463 (M-15), 252/253 & 463 (M-15), 224/225, 280/281 & 463 (M-15), 196/197, 308/309 | 3.83 | 1.33 | 1.36 | 0.68 | |
| 69 | x,x-tetratriacontadiene1 | 3343 | 474 (M+), 96 | tr | 1.45 | 0.23 | ||
| 70 | x,x-dimethyl tritriacontane1 | 3357 | 57, 71, 85 | tr | 0.28 | 0.17 | ||
| 71 | 5,x-dimethyl tritriacontane1 | 3367 | 478 (M-15), 57, 71, 85, 436 | tr | 0.58 | 0.39 | ||
| 72 | Tetratriacontane | 3400 | 478 (M+) | tr | 0.10 | 0.06 | ||
| 73 | 9,19-pentatriacontadiene | 3443 | 488 (M+), 96 | 676 (M+), 173, 271, 311, 357, 409, 455, 535, 582 | 1.24 | 0.27 | 43.19 | 4.76 |
| 74 | x-pentatriacontene1 | 3480 | 490 (M+), 97 | 0.43 | 0.17 | |||
| 75 | x-methyl x-pentatriacontene1 | 3515 | 57, 97, 111 | 0.33 | 0.19 | |||
| 76 | 15-methyl pentatriacontane | 3521 | 491 (M-15), 224/225, 308/309 | 2.05 | 0.64 | 0.49 | 0.28 | |
| 77 | 7,15-dimethyl pentatriacontane | 3558 | 506 (M-15), 112/113, 239, 308/309, 435 | tr | 0.54 | 0.21 | ||
| 78 | 5,17-dimethyl pentatriacontane | 3569 | 506 (M-15), 84/85, 239, 308/309, 464 | tr | 0.30 | 0.20 | ||
| 79 | x,x-heptatriacontadiene1 | 3653 | 516 (M+), 96 | 1.22 | 0.52 |
1The position of the double bond(s) and/or the methyl group(s) could not be determined.