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. 2018 Jan 10;8:332. doi: 10.1038/s41598-017-18706-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Stress and ethanol exposure do not affect electrically-evoked DA signal recovery during aCSF wash following ethanol application. After the final ethanol concentration (220 mM), the brain slices were perfused with aCSF containing no ethanol. There was no significant difference in the recovery of electrically-stimulated DA efflux between any groups after replacing the ethanol solution with aCSF. Data are presented as mean ± SEM and analyzed by a Friedman test. The number of subjects (a single slice per rat) for naïve nonstressed: n = 7; for naïve stressed: n = 10; for ethanol drinking and nonstressed: n = 6; for ethanol drinking and stressed: n = 5.