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. 2018 Jan 10;8:242. doi: 10.1038/s41598-017-18299-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Examples of LC-MS/MS fragmentation spectra of glycan (A) sialylated structure with composition NeuAc₁Hex₁HexNAc₂ [M-H]⁻ ion of m/z 878, (B) LacdiNAc containing O-glycan with composition dHex₁Hex₁HexNAc₃ [M-H]⁻ ion of m/z 936. (C) Blood group H epitope containing glycan with composition dHex₂Hex₂HexNAc₂ [M-H]⁻ ion of m/z 1041. Proposed structures are depicted using SNFG nomenclature⁶³ and fragments of carbohydrates as defined by Domon and Costello⁶⁴.