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. 2017 Sep 15;25(2):229–240. doi: 10.1038/cdd.2017.144

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This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/4.0/

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Elevated antioxidative capacity during osteogenic differentiation. MC3T3-E1 cells were induced to differentiate for 7, 14, and 21 days, and cellular antioxidative capacity was evaluated by testing (a) cellular ROS content, (b) total and mitochondrial carbonyl protein levels, (c) T-AOC, (d) total-SOD activity, (e) SOD2 activity, (f) SOD1 activity, (g) mRNA levels of SOD1 and SOD2, and (h) protein levels of SOD1 and SOD2. Data are presented as the mean±S.E.M. from at least three independent experiments. *P<0.05, **P<0.01 versus relative control