Figure 4.

p53 mediates the effects of MLN4924 on G2/M arrest and apoptosis in hSMCs. (a) Western blotting analysis of Nedd8-cullins, p-H2AX, p-p53, and total p53 in hSMCs treated with vehicle (DMSO) or MLN4924 (MLN). GAPDH was used as a loading control. (b) Quantification of (a). (c) QRT-PCR verification of gene-specific silencing of p53 in hSMCs. NC siRNA: negative control siRNA. (d) Western blotting verification of gene-specific silencing of p53. (e) FACS analysis of cell cycle of hSMCs. (f) Quantification of percentages of hSMCs at different cell cycle phases. (g) TUNEL staining of hSMCs. (h) Quantification of TUNEL-positive cells. (i) QRT-PCR analysis of fas and p21. Cells were transfected with siRNA for 24 h and then treated for 72 h. Scale bar: 300 μm. Each experiment was repeated at least three times. *P<0.05, **P<0.01