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. 2017 Oct 13;25(2):319–329. doi: 10.1038/cdd.2017.160

Figure 5.

Figure 5

p62 mediates the pro-apoptotic effects of MLN4924 in hSMCs. (a) QRT-PCR analysis of gene expression of p62 in hSMCs treated with vehicle (DMSO) or MLN4924 (MLN). (b) Western blotting analysis of p62 in hSMCs treated with DMSO or MLN. GAPDH was used as a loading control. (c) Quantification of (b). (d) Western blotting verification of gene-specific silencing of p62 in hSMCs. NC siRNA: negative control siRNA. (e) TUNEL staining of hSMCs. (f) Quantification of TUNEL-positive cells. (g) Western blotting analysis of cleaved caspase 3 in hSMCs. (h) Quantification of (g). (i) QRT-PCR analysis of gene expression of p62 in hSMCs treated with DMSO or MLN and with NC siRNA or p53 siRNA. (j) Western blotting analysis of p62 and p53. (k) Quantification of p62 western blotting result. For (a–c), cells were treated for 72 h. For other panels, cells were transfected with siRNA for 12 h (d–h) or 24 h (i–k), and then treated for 72 h. Scale bar: 300 μm. Each experiment was repeated at least three times. *P<0.05, **P<0.01, ***P<0.001