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. 2017 Nov 10;25(2):368–379. doi: 10.1038/cdd.2017.166

Figure 3.

Figure 3

Overexpression of TM9SF4 promoted autophagic flux in HEK293 cells. (a and b) Effect of TM9SF4 overexpression on LC3-II level in the presence or absence of bafilomycin A1 (Baf, 10 nM). Shown are representative immunoblot images (a) and data summary (b). (c and d) Effect of TM9SF4 overexpression on RFP-LC3 puncta formation. Shown are LC3 fluorescent signals from representative single cells (c) and data summary (d). The right most panels in (c) were the magnified images of Merge. Scale bar=10 μm. The quantification of RFP-LC3-positive autophagosome was performed using ImageJ. Amino acid starvation was carried out in EBSS for 2 h. Summary data are presented as mean±S.E.M. (n=6 in B; n=5 and >100 cells per experiment in (d). The values in (b) were normalized to β-actin level. *P<0.05; **P<0.01