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. 2017 Nov 10;25(2):368–379. doi: 10.1038/cdd.2017.166

Figure 4.

Figure 4

TM9SF4 inhibited mTOR activity in HEK293 cells. (a–d) Representative immunoblot images (a) and data summary (b–d) showing the effect of TM9SF4-shRNA1 on the expressional levels of phospho-mTOR/mTOR, phospho-4E-BP1/4E-BP1 and phospho-ULK1-S757/ULK1. (e–h) Representative immunoblot images (e) and data summary (f–h) showing the effect of TM9SF4 overexpression on the expressional levels of phospho-mTOR/mTOR, phospho-4E-BP1/4E-BP1 and phospho-ULK1-S757/ULK1. (i and j) Representative immunoblot images (i) and data summary (j) showing that mTOR-siRNA caused LC3-II re-accumulation in lenti-TM9SF4-shRNA1-expressing cells. mTOR-siRNA could effectively knockdown the expression level of mTOR proteins (i and k). Amino acid starvation was carried out in EBSS for 4 and 6 h in (a–d), 4 h in (e–k). Summary data are presented as mean±S.E.M. (n as labeled in the figures). The values in summary data were normalized to total protein levels of mTOR, 4E-BP1 and β-actin, respectively. *P<0.05; **P<0.01