Figure 3.

miR-142-3p regulates neuronal differentiation, presumably via reducing STAU1 expression in SH-SY5Y cells. (a) Mature miRNAs during differentiation in SH-SY5Y cells were quantified by using RT-qPCR. The relative levels of RNAs at day 0 of differentiation were set to 1.0. (b,c) SH-SY5Y cells that were transfected with an miR-142-3p mimic or a control miRNA mimic were differentiated. (d,e) SH-SY5Y cells at day 5 of differentiation that were transfected with an miR-142-3p inhibitor or a control miRNA inhibitor were differentiated for 2 days. Cell lysates were employed in western blots (b,d) and RT-qPCRs (c,e). The relative levels of miRNAs and mRNAs were normalized to the level of U6 snRNA. *P<0.05, **P<0.01