Figure 6.

STAU1 regulates differentiation of mNPCs. (a,b) mNPCs from cortex at E12.5 were differentiated for the indicated number of days. Western blot (a) and RT-qPCR (b) were performed to detect specific mouse (m) proteins (blot) and quantify the endogenous mRNAs and miRNAs (RT-qPCR). The relative levels of miRNAs and mRNAs were normalized to those of U6 snRNA and mGAPDH mRNA, respectively. The relative levels of RNAs at day 4 of differentiation were set to 1.0 in (b). **P<0.01 and *P<0.05. (c) STAU1 expression was upregulated by infection with a retrovirus expressing STAU1⁵⁵-HA₃ and GFP or GFP alone during the differentiation of mNPCs. Western blot was performed to detect expression of the indicated proteins. (d) Same as (c) except that SYP and nuclear (DAPI) staining at day 12 of differentiation were observed under an immunofluorescence microscope. (e) The suggested model illustrates the sequential regulation of Lin28B:miR-142-3p:STAU1 mRNA during neuronal differentiation