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. 2018 Jan 10;8:230. doi: 10.1038/s41598-017-18431-6

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fibrin-based 3D angiogenesis assay. Representative Brightfield and fluorescence images of sprouting MSC-OEC spheroids, cultured in normal and XF conditions for periods of respectively 1, 4, and 7 days; brought under the assay conditions for 72 h. OEC were marked with DiI-Ac-LDL and MSC were labelled with a CellTracker Green Dye. Scale bars correspond to 100 μm. Graphs represent quantitative analyses of the average numbers of sprouts per spheroid and their length, respectively under normal and XF conditions (n = 7, Mann-Whitney test was used to compare the two groups).