Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jan 10;8:256. doi: 10.1038/s41598-017-18433-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Caspases and autophagy are required for CSF-1 or IL-34 induced macrophagic differentiation. (a) Human monocytes were exposed for 2 days to 100 ng/mL CSF-1 or 100 ng/mL IL-34 alone or in combination with either 50 µM qVD or 3 mM 3MA (3-MethylAdenine), which were added 30 min before CSF-1 or IL-34 treatment. The expression of cleaved NPM (NPM*), LC3B and cleaved cathepsin B (CTSB*) was analyzed by immunoblotting. (b) Human monocytes were treated as in Fig. 3a. (c,d) Monocytes were transfected with siRNA targeting LUCIFERASE (LUC), CASPASE-8 (CASP8) or BECLIN (BEC) and exposed for 2 days to 100 ng/mL CSF-1 or 100 ng/mL IL-34. (c) Expression of Caspase-8, Beclin and LC3B was analyzed by immunoblotting. Actin is used as a loading control. Asterisk indicates a cleavage fragment. (d) Differentiation was examined as previously described.