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. 2018 Jan 10;8:255. doi: 10.1038/s41598-017-18305-x

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© The Author(s) 2017, corrected publication 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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LPS-induced protein expression of different chemokines in presence or absence of TLC. Primary human macrophages are pre-incubated with 50 µM TLC for 45 min or were left untreated, and when indicated, LPS (10 ng/ml) was added for another 24 h before the supernatant was collected. (a) Supernatants from macrophages were analysed via Luminex Technology. Secreted LPS-induced chemokines are significantly down-regulated by TLC. (b) Supernatants from macrophages were analysed via Luminex Technology and ELISA and expression of IL-10, IL-2RA, MMP10 and MMP12 were detected. Data are presented as the means ± SEM (n = 4). *P-value ≤ 0.05.