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. 2018 Jan 10;8:393. doi: 10.1038/s41598-017-18763-3

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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In vivo bioluminescence imaging of NG2-fLuc Tg rats during the first postnatal hair cycle. (A) Bioluminescence images of male Tg rats from P19 to P38 during the first postnatal hair cycle; black squares in each image show regions of interest (ROI). Haematoxylin-stained images of skin from Tg rats visualized by bioluminescence imaging at each time point; scale bars; 500 μm. (B and C) Quantification of bioluminescence signals from ROI in the dorsal surface skin of male (N = 14, blue diamonds in B) and female (N = 8, pink diamonds in C) Tg rats during the first postnatal hair cycle. Data from each animal were presented as means ± SD. *P < 0.05 and **P < 0.01; compared with telogen phase (P23 in B or P24 in C).