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. 2018 Jan 10;8:251. doi: 10.1038/s41598-017-18355-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Separation and partial purification of an actinoporin-like hemolysin from Stylophora. In the chromatograms, the shaded areas represent active fractions that were pooled and submitted either to re-chromatography (after anion exchange) or two tandem mass spectrometry (after hydrophobic interactions chromatography). The straight grey lines represent the gradient of salt used for chromatography. (a) Separation of Stylophora crude extract by anion exchange chromatography on a Q- sepharose FF 18.5 ml column. (b) Separation of pool Q-2 from panel A, above, on a Hydrophobic Interactions Phenyl- Sepharose HP 7.8 ml column.