Supplementary Figure S1.

MiR-34a levels in cholangiocarcinoma cells transfected with miR-34a mimic/inhibitor or scramble control. A: The expression of miR-34a in CCLP1 and SG231 cells transfected with miR-34a mimic or scramble control. B: The levels of miR-34a in TFK1 cells transfected with miR-34a inhibitor or scramble control. The miR-34a level was measured by quantitative RT-PCR analysis. RNA U6 small nuclear 2 was used as internal control. Data are expressed as means ± SEM. ∗P < 0.05, ∗∗∗P < 0.001 versus ctrl-mimic transfected cells; ^†P < 0.05 versus anti-NC. Anti-NC, antisense-negative control; Ctrl, control.