Figure 4.

Quantification of immune infiltrates into enlarged versus control prostates. To measure changes in immune cell populations after prostatic enlargement, we stained and quantified the presence of T cells via CD3 (A), B cells via B220 (B), and macrophages via F4/80 (C). Data from 12- and 15-week time points, after significant prostatic enlargement, were combined for increased statistical power. We observed no statistically significant change in T-cell, B-cell, or macrophage populations on prostatic enlargement. Representative images of immunostaining are shown, each from the dorsal lobe. Arrowheads represent positive cells for immunohistochemical stain of corresponding markers. Original magnification, ×40 (A–C). T control, testosterone alone; T+E, testosterone and estradiol.