Table 1.
Relative Expression in Cultured RPE versus Native RPE Cells
| Gene Symbol | Gene Name | NCBI Accession No. | Relative Expression (2−ΔΔCt) | p Value |
|---|---|---|---|---|
| Downregulated Genes | ||||
| BEST1 | bestrophin 1 | XM_003353833.1 | 0.043 | <0.0001 |
| BMP4 | bone morphogenetic protein 4 | NM_001101031.2 | 0.132 | <0.0001 |
| CACNB2 | calcium channel voltage dependent beta 2 | XM_003482816.1 | 0.161 | <0.0001 |
| CLDN19 | claudin-19 | NM_001160084.1 | 0.580 | 0.0006 |
| CRX | cone-road homeobox | XM_003127265.1 | 0.001 | <0.0001 |
| CTSD | cathepsin D | NM_001037721.1 | 0.108 | <0.0001 |
| DCT | dopachrome tautomerase | NM_001025227.1 | 0.001 | <0.0001 |
| FADS1 | fatty acid desaturase 1 | NM_001113041.1 | 0.526 | 0.0002 |
| ITGB5 | integrin, beta 5 | NM_001246669.1 | 0.427 | <0.0001 |
| KCNJ13 | potassium inwardly rectifying channel subfamily J,13 | XM_001926506.3 | 0.033 | <0.0001 |
| KRT8 | keratin 8 | NM_001159615.1 | 0.364 | <0.0001 |
| KRT18 | keratin 18 | XM_003126180.3 | 0.058 | <0.0001 |
| LHX2 | lim homeobox protein 2 | NM_001170519.1 | 0.452 | 0.0004 |
| LRAT | lecithin-retinol acyltransferase | NM_001244920.1 | 0.014 | <0.0001 |
| LRP8 | apolipoprotein e receptor | NM_001199891.1 | 0.365 | <0.0001 |
| MERTK | c-mer proto-oncogene tyrosine kinase | XM_003124812.3 | 0.052 | <0.0001 |
| MKI67 | marker of proliferation ki-67 | NM_001101827.1 | 0.338 | <0.0001 |
| OTX2 | orthodenticle homolog 2 | XM_003353491.1 | 0.422 | <0.0001 |
| PMEL | premelanosome protein | XM_003481626.1 | 0.018 | <0.0001 |
| RDH10 | retinol dehydrogenase 10 | XM_001928082.3 | 0.031 | <0.0001 |
| RPE65 | retinal pigment epithelium 65 kDa | XM_003127931.2 | 0.088 | <0.0001 |
| SERPINF1 | serpin peptidase inhibitor | NM_001078662.1 | 0.207 | <0.0001 |
| SLC16A8 | solute carrier family 16, member 8 | XM_003126028.1 | 0.023 | <0.0001 |
| SLC24A5 | solute carrier family 24, member 5 | XM_003121523.1 | 0.261 | <0.0001 |
| SLC39A12 | solute carrier family 39, member 12 | XM_003130728.1 | 0.023 | <0.0001 |
| TRPM3 | transient receptor potential cation channel | XM_001925032.3 | 0.551 | 0.0008 |
| Upregulated Genes | ||||
| SLC16A3 | solute carrier family 16, member 3 | XM_003357925.1 | 13.193 | 0.0002 |
| SLC16A12 | solute carrier family 16, member 12 | XM_001928811.2 | 2.885 | <0.0001 |
| TYRP1 | tyrosinase related protein 1 | NM_001025226.1 | 48.561 | <0.0001 |
Relative expression (2−ΔΔCt) of genes in cultured RPE cells normalized by the expression in native RPE cells. Data normalized to GAPDH. Statistical analysis: multiple t test and Holm-Sidak method, with alpha = 1.000%, n = 3 biological triplicates.