Table 2.
Relative Expression in Cultured RPE Transduced with AAV-Otx2 and AAV-Otx2L versus Control Transduced RPE Cells
| Gene Symbol | Gene Name | Relative Expression (2−ΔΔCt) |
p Value | |
|---|---|---|---|---|
| AAV-OTX2 | AAV-OTX2L | |||
| CACNB2 | calcium channel voltage dependent beta 2 | 1.59 | – | 0.002 |
| CLDN19 | claudin-19 | 0.42 | 0.41 | 0.003 |
| CRX | cone-road homeobox | 18.88 | 18.12 | <0.0001 |
| DCT | dopachrome tautomerase | 8.68 | 4.77 | <0.0001 |
| KCNJ13 | potassium inwardly rectifying channel subfamily J,13 | 9 | 6.77 | <0.0001 |
| KRT18 | keratin 18 | 2.45 | 2.68 | <0.0001 |
| MKI67 | marker of proliferation ki-67 | 1.67 | – | 0.0005 |
| RDH10 | retinol dehydrogenase 10 | 3.36 | 2.87 | <0.0001 |
| RPE65 | retinal pigment epithelium 65 kDa | – | 0.42 | 0.003 |
| SLC16A8 | solute carrier family 16, member 8 | 2.52 | 2.79 | <0.0001 |
| SLC16A12 | solute carrier family 16, member 12 | 4.91 | 3.06 | <0.0001 |
| SLC39A12 | solute carrier family 39, member 12 | 0.44 | 0.42 | <0.004 |
| TYR | tyrosinase | 13.42 | 10.92 | 0.0008 |
| TYRP1 | tyrosinase related protein 1 | 2.66 | 2.48 | <0.0001 |
Relative expression (2−ΔΔCt) of genes in cultured RPE cells transduced with AAV-Otx2 and AAV-Otx2L normalized by the expression in control cells (AAV-GFP-transduced RPE). Data normalized to GAPDH. Statistical analysis: two-way ANOVA and Dunnett test, with alpha = 1.000%, n = 3 biological triplicates.